The Excelitas pco.flim camera system consists of the pco.flim camera and pco.flim laser, with a complete frequency synthesizer, which is required for the generation of the modulation signals in the frequency domain. The pco.flim series features a high frequency modulated CMOS image sensor that enables pixel-wise measurements of sample luminescence lifetime distributions in frequency domain suitable for fluorescence lifetime imaging. At a maximum speed of 10 Hz, full luminescence lifetime distributions can be measured at 1 Mpixel resolution. With flexible coupling and easy setup, the pco.flim is an ideal choice for wide-field FD-FLIM instruments and suitable for a variety of biomedical applications requiring a large frame and high-speed acquisition.